Separation and determination of sulfamethoxazole, trimethoprim and metoclopramide hydrochloride by RP-HPLC method in pure and in Pharmaceutical formulations
Mustansiriyah University, College of science, Department of chemistry, Iraq
Mustansiriyah University, College of science, Department of chemistry, Iraq
Mustansiriyah University, College of science, Department of chemistry, Iraq
Mustansiriyah University, Collage of Basic Education, Science Department/Iraq
Abstract
A very accurate, fast and simple method for separating and determine pharmaceutical compounds sulfamethoxazole (Sulfa), trimethoprim (Tri), and metacalpermide hydrochloride (Meta). The separation process was performed by HPLC on a C18 stationary phase column. The method was developed and validated. The mobile phase is a mixture of acetonitrile buffer 60.40 under isocratic elution. The flow rate was 1.2 mL/min, and the optimum temperature for the separation was 45 °C. The analysis time is up to 4 minutes with retention times of 2.204, 2.873 and 4.983 minutes for the studied pharmaceutical compounds. The linearity was in the range of 0.1-100 µg/mL. The developed method was tested for the quantitative determination of pharmaceutical compounds in its pure form and in different commercial pharmaceutical formulations with less interference of additives.
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